How dogs assist in the detection of Apollo caterpillars

People discovered the sensitivity of a dog’s nose thousands of years ago, initially using it for hunting. Fast forward to present, and these animals have evolved into valuable members of our society, serving in police, customs, military, and rescue missions. Dogs have an incredible sense of smell that is a million times more sensitive than ours. It is related to the numerous scent receptors in their noses and the highly developed nasal processing centers in their brains. Dogs not only use their noses to detect scents, but they can also differentiate between various odours with amazing accuracy. 

Thanks to that, dogs have become indispensable in research and conservation projects over the last 30 years. These sniffer dogs have been trained for a variety of tasks ranging from tracking wolves, lynx, wildcats, or bats to identifying carcasses (dead bodies), litter, and even targeting small insects. The possibilities are almost limitless, as long as the target object emits a smell, dogs can track it down. While this type of use of dog is common in the USA, Canada, Australia, and New Zealand, the use of nature or species protection dogs remains relatively unknown in Europe. 

And #LIFEApollo2020 is proud to be one of these scientific projects!

What: Our focus is on collecting data about Parnassius apollo with detection dogs playing a key role in mapping caterpillar population across selected sites. These dogs ensure precise and non-invasive data collection by identifying unique odors associated with Apollo caterpillars and their habitats.

How: Dogs undergo a special training, that teaches them to recognize the unique odors associated with Apollo caterpillars. At first, the dog sniffs the live caterpillars and gets a treat for it. Then, tea bags scented with the caterpillar odor are introduced as a game element. The dog learns to associate finding the tea bags with getting a reward. As the training progresses, the dog practices in different places to get better at identifying the caterpillar scent. This simple but effective method helps the dog find the Apollo butterfly caterpillar in the wild.

When: Fieldwork usually takes place from March/April to June, when the caterpillars hatch from the eggs and crawl around. 

Why: Dogs with their great sense of smell easily overtake humans in finding and detecting targets. The caterpillar monitoring, facilitated by the human-dog team guides us in conservation efforts, allowing us to make informed decisions on preserving this endangered species in its natural habitat.

October 2023 was a month of conferences!

In October, LIFE Apollo2020 was presented at two significant conferences!

The congress took place in Heraklion, Crete, from the 16th to the 20th of October. This international event was an important meeting for more than 1000 entomologists and enthusiasts from the European Union, as well as representatives from overseas (UK, USA, Australia). The diversity of attendees transformed ECE 2023 into a global gathering, enabling a rich exchange of ideas and experiences.

The congress offered a truly comprehensive program, involving thematic sessions such as invasion biology and climate change, ecology and behavior, biodiversity and conservation, and much more. Workshops, poster presentations, excursions and hundreds of lectures provided the participants with the unique opportunity to learn about the latest research and developments in the field.

Our team member Tomáš E. Vondřejc held a scientific poster presentation on “Conservation of Parnassius apollo in Poland, Czech Republic and Austria under the theme of “Biodiversity and Conservation“. The project LIFE Apollo2020 made a great impression, capturing the attention of dozens of individuals with a keen interest in the conservation of P. apollo butterflies. Particularly captivating were the specifics of Apollos’ breeding farms and their operation. The innovative approach of using dogs for butterfly identification sparked broad interest as well, opening possibilities for the application of this measure in various conservation projects.

Throughout the congress, Tomáš had the opportunity to engage in conversations with numerous experts in the field, notably those involved in other LIFE projects with similar focuses, such as SouthLIFE and LIFE for Pollinators. The Project LIFEApollo2020 also caught an attention of the CINEA representatives from the European Commission, leading to a detailed discussion about the project. The outcome was successful, with LIFE Apollo2020 being pointed out as an exemplary case of good practice in their presentation, positioning it as one of the most outstanding species-focused projects for implementation.

The Apollo Project team is very grateful to be a part of ECE2023 and looks forward to future opportunities.

The LIFEApollo2020 team from Poland and Czechia participated from 19th to 20th October in the scientific conference ‘Apollo butterfly – research, protection, and monitoring’ in Červený Kláštor, Slovakia. Thanks to the event organized by Pieninský národný park (PIENAP) and Pieniński Park Narodowy (PPN), we had the opportunity to present our project to a broader international community of scientists and practitioners.

Our team delivered three presentations in total. Tomasz Suchan from Instytut Botaniki im. W. Szafera Polskiej Akademii Nauk w Krakowie discussed ‘Genetic research of the Apollo butterfly as part of the LIFE Apollo2020 project’, David Číp, representing Skupina JARO shared insights on ‘Experience in the protection and breeding of the Apollo butterfly within the JARO Group‘, and lastly, Dariusz Kuś from Karkonoski Park Narodowy addressed ‘Active protection of the Apollo butterfly in Karkonoski National Park – the LIFE Apollo2020 project’.

The conference offered an excellent platform for engaging in discussions, exchanging experiences, and gaining knowledge about conservation initiatives in various regions of Europe and the world. Our collaboration with PIENAP and PPN holds significant importance, as we share common objectives, enabling us to mutually enhance our projects and effectively protect Parnassius apollo and its natural habitats.

We would like to express our deep gratitude to the management, Mr. Vladimír Klc, the director of PIENAP, Mr. Michał Sokołowski, the director of PPN, and Ms. Iwona Wróbel, deputy director, as well as the teams of both National Parks for their invitation and the organization of this event. We also appreciate the dedicated moderators, Mr. Paweł Adamski and Mr. Ludomir Panigaj, for facilitating productive communication among participants.

Decline of Parnassius apollo

Parnassius apollo, commonly known as the Apollo butterfly, is a beautiful and iconic species of butterfly found in Europe and Asia. Unfortunately, this species is on a rapid decline due to several factors, including habitat loss, climate change, and over-collecting.

The Apollo butterfly has a very specific habitat requirement, which is high altitude meadows and rocky slopes with specific plant species for feeding and breeding. However, these habitats are under threat from human activities such as tourism, agricultural expansion, and infrastructure development. As a result, the population of the Apollo butterfly is decreasing at an alarming rate. 

Factors of the decline 

Parnassius apollo belongs to the most attractive butterfly and very often appreciated not onlyby scientists but also visitors. Therefore, decline of this butterfly species is so sensitive and painful. Decline of Parnassius apollo, particularly in a central Europe is dated back already since the 19 centuries. 

Observations of gradual decline, some environmentalist even use the word extinction, of this butterfly in Europe identified numerous cases, proved that decline is very much due to combined negative impact on Parnassius population. 

There are several major factors contributing to the decline of the Parnassius apollo population. One of the main factors is habitat loss. The main reason for that are human activities such as tourism, infrastructure development, and agricultural expansion. 

Habitat requirements

The Apollo butterfly has very specific habitat requirements, including high altitude meadows and rocky slopes with certain plant species for feeding and breeding. When these habitats are destroyed or degraded, the butterfly population declines.

Habitat loss is one of the main factors to the decline of the Parnassius apollo
Combination of habitat loss, climate change, over-collecting, and pollution are the major factors contributing to the decline of the Parnassius apollo

Climate change

Climate change is another major factor affecting the Apollo butterfly population. The butterfly’s life cycle is closely tied to the timing of the availability of host plants and pollinators, which can be disrupted by changes in temperature and precipitation patterns. As a result, the butterfly may not be able to complete its life cycle and reproduce successfully.

Over-collecting 

Over-collecting of the Apollo butterfly is also a contributing factor to its decline. Some people collect the butterfly for commercial purposes or for private collections, while others collect it for scientific research. This can have a significant impact on the population, especially if it is not done sustainably.

Pesticide use

Finally, the Apollo butterfly is also affected by pesticide use and other pollutants in the environment. These substances can be toxic to the butterfly and its host plants, and can disrupt the delicate balance of the ecosystem.

Overall, a combination of habitat loss, climate change, over-collecting, and pollution are the major factors contributing to the decline of the Parnassius apollo population. It is important that effective conservation efforts are put in place to address these issues and protect this beautiful species.

Major groups of factors

Experts agreed on the three major groups of factors such as: 

a) natural factors including long-term climatic changes, habitat succession, and short-term weather anomalies; 

b) anthropogenic factors that include broad impact of industrialization and butterfly over-collecting; 

c) intrapopulation factors that include genetic erosion and behavioural changes. 

Habitat loss is undoubtedly the most destructive for Apollo’s long-term survival. There are several interesting researches and monitorings which provide very important information concerning causes of extinction of numerous butterflies in their biotopes in Europe. 

Conservation efforts

To help protect the Apollo butterfly, several conservation efforts have been implemented (one of them is our LIFE Apollo2020 project). These include habitat restoration, captive breeding, and protection of important sites. However, these efforts must be implemented on a larger scale and in a coordinated manner involving multiple stakeholders, including governments, NGOs, researchers, local communities, and individuals.

Conclusion

In conclusion, urgent action is needed to prevent the extinction of the Apollo butterfly. Conservation efforts must be comprehensive and well-coordinated to ensure the long-term survival of this beautiful and important specie

Very useful on this aspect is report developed by the expert of IUCN already two decades ago. These data provided important baseline for any kind of follow up researches and management measures to support survival rate of this rare butterfly. This work provides various protective measures that were, or should be undertaken to stop further Parnassius apollo decline. 

Climate change is a significant threat to the survival of Parnassius apollo. The altered environmental conditions and increased frequency of extreme weather events can negatively impact their habitats, food sources, and overall health and survival. 

LIFE Apollo2020 is present at the XII European Congress of Entomology 2023!


The XII European Congress of Entomology 2023 (ECE 2023) is set to take place in Heraklion, Crete, from the 16th to the 20th of October. This international event is a significant gathering for entomologists and enthusiasts, offering a space for discussing critical entomological research and conservation efforts. 

You might wonder what entomology is? It is the scientific study of insects, a field of biology that explores a fascinating and diverse world of these little creatures. Insects, representing a vast majority of known species on Earth, play a crucial role in ecosystems, agriculture, and human life. From pollinating crops to recycling organic matter, insects have a big impact on the world we inhabit.

Among the participants of the Congress is our team member Tomáš Ernest Vondřejc, a dedicated zoologist from the Education and Information Centre White Carpathians, representing the LIFE Apollo2020 project. He is going to hold a scientific poster presentation on “Conservation of Parnassius apollo in Poland, Czech Republic and Austria (Project LIFE APOLLO2020)” under the theme of “Biodiversity and Conservation“.

Date: Thursday, 19th October 2023 (Poster Session IV)
Location: Cultural Conference Center of Heraklion, Crete, Greece (Poster Area is on Level 1)
You will find the Detailed program of the congress here.

Tomáš Ernest Vondřejc is part of the LIFE Apollo2020 project team, and his work is important in ensuring the successful preservation of Parnassius apollo butterflies and their habitats. His responsibilities range from conducting in-depth research to organizing field trips and implementing practical conservation strategies.

The LIFE Apollo2020 project is looking forward to be represented at the ECE 2023, where it will have the opportunity to network with other enthusiastic entomology professionals and exchange knowledge with them.

For more information, please visit the official website of ECE 2023.

Parnassius apollo: The umbrella species guarding biodiversity

When it comes to protecting our planet’s valuable biodiversity, it sometimes takes a hero to defend the masses. The Parnassius apollo is a champion in the fascinating world of butterflies. The Apollo butterfly, often referred to as a “umbrella species,” plays a critical role in protecting not only its own species but an entire environment rich with lesser-known species. In this article, we’ll focus on why the conservation of Parnassius apollo is so crucial and how it acts as a bioindicator, offering valuable insights into the health of its habitat.

The umbrella effect

Imagine a majestic forest, vibrant with life. Within it, an umbrella opens, shielding the delicate flora and fauna beneath from the harsh elements of habitat loss and environmental degradation. This metaphorical umbrella is the Parnassius apollo, a butterfly species that holds a vital position in the web of life.

When we say “umbrella species,” we mean that by looking after the Parnassius apollo, we actually keep many other animals and places safe too. We often talk a lot about saving big animals like tigers and pandas, but small creatures like the Apollo butterflies need our help too.

The domino effect of conservation

By focusing on the preservation of the Parnassius apollo and its habitat, we unintentionally protect countless other species living in the same ecosystem. This butterfly’s presence is indicative of a healthy and intact environment, with thriving populations of plants, insects, and other organisms that rely on the same resources.

The Parnassius apollo acts as an umbrella species, not only for the protection of biodiversity but also for the preservation of habitat mosaics. These mosaic-like habitats are composed of various interconnected ecosystems, creating a rich and diverse landscape. By conserving the species itself, we, in turn, protect other species and habitats associated with it.

Many butterfly species benefit from habitat conservation measures taken to protect the Apollo. A significant number of these butterflies find themselves listed in Red Books of Animals and other rare and endangered species lists, highlighting the critical importance of the Apollo’s preservation.

A sensitive bioindicator

Beyond its role as an umbrella species, the Parnassius apollo serves as a remarkable bioindicator. A bioindicator is a species whose status provides valuable information about the overall health of its ecosystem. In the case of Parnassius apollo, it proves to be particularly sensitive in this regard, making it an invaluable asset in environmental monitoring.

One of the specific areas where the Parnassius apollo excels as a bioindicator is in the monitoring of xerothermic biotopes—ecosystems characterized by hot, dry conditions. These habitats are under constant threat due to climate change and human activities. As an indicator species, the Parnassius apollo can tell us a great deal about the quality and health of these fragile environments. If the butterfly thrives, it suggests that the ecosystem is healthy and stable, while a decline in its population signals potential problems.

Protect Apollo, protect our planet

In a world where nature faces many challenges, the Parnassius apollo stands out as a true hero. It’s not just looking out for itself but also for a whole community of other creatures and the places they call home. And, it’s also a smart detective, helping us understand how our environment is doing. By taking care of the Apollo, we’re not just helping one species; we’re making sure that the amazing web of life on Earth remains strong and healthy. This butterfly helps us protect other animals and special places like sunny meadows and rocky grasslands. By doing this, we’re making the world a better and more diverse place for the future.

Parnassius apollo history and trophic preferences

Parnassius apollo belongs to a group of butterflies, which presence in Europe has a long history. We need to take a look into the past if we want to get to know the roots of this beautiful species. We would have to travel back to the time period called the Neogene.

The Neogene is a geological period, which informally is divided into either the Upper Tertiary or Late Tertiary. This period lasted from 20.6 to 2.6 million years ago, to the beginning of the present Quaternary Period. The Quaternary Period spans from the Neogene to today. 

More on the topic: Apollo in winter

Glacial and interglacial periods 

During the long period of the Neogene, the land was heavily glaciated. Glaciers appeared and retreated several times. Those periods when glaciers retreated are known as interglacial episodes. 

These periods of warm climate provided an opportunity for the animal and plant life to develop, to later be swept away by the glacier and then develop again and again. This process heavily impacted butterflies, including Parnassius apollo

Diversity of life

Research revealed that during these long periods of interglacial episodes, despite the fact that all life was swept out by huge glaciers, it repeatedly revived. This created remarkable diversity again and again. The result of this process was the enormous diversification of life.

For example, Parnassius apollo developed into more than 200 identified subspecies, which mainly inhabit grassland environments in the lowland and mountainous areas throughout Europe. 

Sedum plant provides food for many species of butterflies, including Parnassius species

Parnassius apollo trophic preferences

Food is a vitally important precondition for the favourable status of Parnassius species. According to trophic preferences, two main groups of Parnassius developed. One group that prefers Sedum telephium, and another that feed mainly on Sedum album.

Sedum is a large genus of flowering perennial plants. Sedum species are herbs and have fleshy leaves and stems, which store water very well. This group of plants contains up to 400–500 species. Even today, Sedum provides food to many species of butterflies, including Parnassius species.

Conclusion

For the protection of the individual species or the entire Parnassius genus of butterflies, it is important to know what their key habitats and food needs are. This knowledge could help us identify what kind of ecosystems and biomes have been removed due to human activity and why food is not available for these butterfly species. In the following steps, activities during the restoration process should focus on recovering these extinct biomes.

The Parnassius apollo, commonly known as the Apollo butterfly, needs a very specific diet requirement for its survival and reproduction. Its larvae rely solely on specific Sedum plant species. This specialized diet is crucial for the survival and development of the Apollo butterfly throughout its life cycle. This dependence on particular plants underscores the delicate ecological relationships and the importance of preserving the habitats that support these unique species.

Vlado Vancura
European Wilderness Society

#followapollo and the efforts of our team! Combined skills in breeding, conservation of habitats, research, environmental education, and project management constitute a great combination for the success of our LIFE project

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Training on the Apollo butterfly monitoring in Pieniny Mountains

As a part of the field monitoring training from July 19th to 20th, representatives from the Naturalist’s Club, Karkonosze National Park, and the Władysław Szafer Institute of Botany of the Polish Academy of Sciences visited the habitats of the local population of Apollo butterflies . It was possible thanks to the hospitality of Pieniny National Park.

Under the guidance of specialist Prof. Paweł Adamski form Polish Academy of Sciences, we learned how to properly conduct species monitoring in the field, how to spot and mark Apollo butterflies, and how to accurately record monitoring data.

Together with Prof. Adamski, we visited three key monitoring sites for the Apollo butterfly. This allowed us to observe and experience working in challenging habitats associated with steep mountain slopes.

During the training, we gained valuable insights into the habitats of the Pieniny population. We further enriched this knowledge during visits to two different habitats while being guided by representatives of Pieniny National Park.

As part of Pieniny National Park’s warm hospitality, we learned about the history of Czorsztyn Castle and the basics of oscypek production (a traditional Polish scalded-smoked cheese with a protected-designation-of-origin status) in traditional shepherd’s hut. Conversations and exchange of experiences related to Apollo butterfly breeding were also an integral part of the visit.

Mr. Tadeusz Oleś, the long-term guardian of the butterfly population and breeding success, shared insights about the population and breeding achievements. Experience exchange always brings surprises and fosters the search for common solutions to ensure the species’ safety and continuity.

The training itself, in such picturesque natural surroundings resultd in a lasting significance for collaboration and brought numerous benefits to breeders. It equipped them with a more confident approach to this year’s and future years’ monitoring efforts.

Acknowledgments

We would like to thank the Pieniny National Park for hosting us with warmth and the commitment of your employees. This allowed us, the LIFE Apollo2020 team, to be guests of the magnificent Pieniny National Park.

Our heartfelt thanks go to the Park’s Director, Mr. Michał Sokołowski, and Ms. Iwona Wróbel for their kindness and for making it possible to orgainze the visit. We deeply appreciate Mr. Bogusław Kozik, Mr. Jacek Berezicki, Mr. Paweł Adamski, Ms. Małgorzata Braun-Suchojad, Mr. Stanisław Złydaszyk, and Mr. Mateusz Dziurny for discussions on Apollo butterfly protection, reintroduction, and monitoring details. Special thanks to Mr. Tadeusz Oleś for guiding us through the breeding facility.

Partner Meeting in Austria

In June, the LIFE Apollo2020 project team gathered for a partner meeting, this time in Austria. The main goal of this event was visiting the habitats of the Apollo butterfly. In Austria, there are still some habitat sites where Parnassius apollo butterflies fly and its populations exist. 

Two entire days were spent for visiting the intact habitats, during which a few imagines were spotted. A lepidopterist (an expert specializes in studying butterflies) was present to guide the team around the sites. This meeting turned out to be very important since breeders from various countries came together to share their expertise.

Austria’s Apollo habitats provided an excellent example for the entire project team. For the success of the LIFE Apollo2020 project, it was very important to visit the sites bacause this allows a better and crucial understanding of functionality of existing habitats in other countries. Therefore, by observing where the Parnassius apollo likes to live, the selection of reintroduction sites will be much easier. 

The sites visited around Austria differ greatly and exhibit unique characteristics. Some are close to settlements, others close to train tracks and yet others are located on high mountains and steep rocks. The differences between the habitats are striking and incredibly interesting because they show that the Apollo is very well adaptable to many different environments as long as there is sun, stonecrops (Sedum sp.) for the caterpillars and nectarous plants for imagines. The project team was pleased to conduct this fruitful and interesting meeting while monitoring methods were discussed and exchanged between breeders and scientists. Acquired experience and knowledge will be used to improve the project.

Here are some photos of the various habitats and the magnificent Parnassius apollo itself.

New Breeding Farms Established

In recent months, a project breeding station has been built on the land of an organic farmer near the village of Blatnička in the foothills of the White Carpathians in the Czech Republic. The station was built by the project partner Bílé Karpaty Education and information centre and although some internal elements are still unfinished, it will serve as a home for the first few fertilised Apollo butterfly stations in the coming days. 

Another breeding farm was constructed in Uniemyśl, in the Stone Mountains in Poland by the project partner KP. The construction was finalised at the end of May, after the team had been using a portable breeding tent.

Breeding Farm for Plant Cultivation

So far, the interior of the breeding station has been used for the cultivation of the main food plant, which is the great stonecrop, as well as for the caterpillars of the Apollo butterfly. In the future it will possibly also be used for the cultivation of other food plants. Recently, we have also planted large stonecrop plants next to the breeding station itself, and we have sown two species of cornflowers adjacent to it, which will serve as nectarous plants for the adult butterflies. All plants (both food and nectarous plants) come from our own collections from the White Carpathians. In fact, some of the plants will be used to seed the case for planting at the project sites, and we make every effort to plant only genetically native plants in our project area.

Construction in progress

Breeding Farm in Poland

Breeding started in 2022 in a temporary portable tent. From the breeding farm in Karkonoski National Park we received 209 eggs, from which we eventually obtained 40 butterflies. We obtained 2866 eggs from our own breeding, from which we have about 1600 caterpillars in the current season. The permanent breeding tent was completed an the end of May. The structure of the building is wooden and refers to the body of the historic Field Station building. We moved the caterpillars to newly finished home. Soon the first pupae will appear, and later part of the tent will turn into an enclosure for butterflies.

In 2022, plant seedlings (mostly Cirsium genus) were obtained in the field. We have started establishing a base of nectariferous plants (host plants for imago) in the garden of the field station in Uniemyśl, as well as farming of Sedum maximum (host plant for caterpillars) in the Forestry Nursery of the Kamienna Góra Forest District in Krzeszów and in the garden of the Field Station in Uniemyśl. The resulting base will feed food plants to our breeding farm. The floor of the breeding tent is lined with humus. Inside and around the tent we will create a garden of nectar-producing plants and host plants for caterpillars.

Construction progress in Poland

Project Milestone

Since there is no water source near the breeding station, we use rainwater that falls on the roof of the breeding station for watering. The water is collected in a large 1000 l container. For the project’s progress, the breeding farms are very important. They are milestones that we have already reached. Now, the project has at least one breeding station in every country. 

Two Apollo Gardens built

Recently, an Apollo Garden was built in an Austrian secondary school. European Wilderness Society, one of the project partners, organised this activity and contributed to the construction itself. The school pupils were also very excited to join and help with the physical field work. Apollo gardens are part of our LIFE Apollo2020 project. They are special gardens containing food plants especially for the caterpillars, but also for the butterflies. 

Another garden was built recently by the lead partner KPN in Poland, in coordination with the project partner KP. In combination with that, KPN and KP also organised a lecture at the Nature Educational Centre to which local communities were invited. Some locals also stayed on for helping with the construction of the Apollo Garden and planting butterfly food plants. Some fun activities were also organised, like painting rocks and making seed cards. For that and for the Apollo Garden, a special mix of flowers was created by KPN and KP. In the course of the project, there will be built many more Apollo Gardens.

What is an Apollo Garden?

Apollo Gardens are an important element of the LIFE Apollo2020 project. They are specially created garden plots with food plants for the Parnassius apollo butterfly and caterpillar. Some of them will be built at schools but others are also going to be installed at other public spaces like national parks. In order to support the survival and recreation of Parnassius apollo, the gardens are built in suitable butterfly habitats or in the vicinity thereof. 

The most important plant for the apollo caterpillar is the sedum, which is of course also planted in the Apollo Garden. Sedum plants enjoy a rocky environment, so after planting them, we also added some rocks.

Building process

With the help of the active students, the Apollo Garden was quickly built – even in the scorching sun. We were quite satisfied with the work, and so were the students and the teacher as well as our external butterfly expert. 
In addition, to provide food for the butterflies we sowed some seeds for future Parnassius apollo (and other butterflies) to feed on.

Workshops conducted

Besides the Apollo Gardens, we also conducted some school workshops in Austria; the workshops focused on butterflies, including the Parnassius apollo. The students were very interested in learning about the important pollinators from our butterfly expert. They learned about the fascinating life cycle of the Parnassius apollo and how it transforms itself from egg to caterpillar to pupa to finally become the beautiful butterfly it is. Moreover, they discovered in which habitats and environments the apollo likes to live. To also create butterfly gardens at their homes, the students learned about the plants they should keep to make it more butterfly-friendly.